Recipes+for+Benedict

Sodium carbonate is soda ash, or washing soda (old Arm and Hammer product). Typcially found as pentahydrate, but Benedict's calls for anhydrous, so need to adjust. Copper sulfate is an algacide for swimming pools. Potassium sodium tartrate crystals can be grown by heating and then cooling a solution of cream of tartar (a common spice) and washing soda (sodium carbonate). Citrate is common and can be sodium or potassium for Benedicts. Potassium thiocyanate and potassium ferricyanide are available as photography supplies.

Reagents used for testing foodstuffs
[|Biuret reagent] This is used to test for the presence of [|protein]. There are two recipes the first consists of two reagents Biuret A and Biuret B. For the second (Quantitative) recipe, in about 600mL of distilled water, dissolve in order 3g of [|copper (II) sulfate] · 5H2O, 5g of [|potassium iodide], 9g of [|potassium sodium tartrate].4H2O, and 8g of [|sodium hydroxide]. Make the dissolved solids to 1 litre. Label the solution **IRRITANT** [|Benedict's reagent] is used to test for [|reducing sugars]. It has two recipes. Benedict's qualitative and Benedict's quantitative. [[|edit]]
 * A is 0.1M [|sodium hydroxide] or [|potassium hydroxide] the bottle should be labelled **CORROSIVE**.
 * B is 0.01M [|copper (II) sulfate] solution

Benedict's Qualitative Reagent
The reagent is made up by first dissolving 173g of [|sodium citrate] and 100g of [|anhydrous] [|sodium carbonate] in about 600ml of distilled water. Then 17.3g [|copper (II) sulfate] · 5H2O is dissolved in about 100ml of distilled water. The two solutions are then mixed together and when cool are made up to 1L with distilled water. [[|edit]]

Benedict's Quantitative Reagent
In about 600ml of hot water dissolve In about 100ml of water dissolve When the solutions have cooled, mix them together stirring constantly. Add [[|edit]]
 * 200g of sodium citrate
 * 75g sodium carbonate
 * 125g [|potassium thiocyanate]
 * 18g of copper (II) sulfate · 5H2O
 * 5ml of 5[|%] [|potassium ferrocyanide] then make up to 1L.

Iodine Solution
Recipes vary but 1g of iodine plus 1g of [|potassium iodide] in 100mL water is suitable. Dissolve the iodide then the iodine.
 * [|Iodine solution] is used to test for [|starch].